TRIM32 antibody (N-Term)

Catalog No. ABIN2780865

Cited in 2+ publications. The Rabbit Polyclonal anti-TRIM32 antibody (ABIN2780865) specifically detects TRIM32 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Guinea Pig samples.

Quick Overview for TRIM32 antibody (N-Term) (ABIN2780865)

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM32 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM32 Antibody

Binding Specificity: N-Term

Purpose: TRIM32 Antibody - N-terminal region

Sequence: MAAAAASHLN LDALREVLEC PICMESFTEE QLRPKLLHCG HTICRQCLEK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TRIM32. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM32

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8114668

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRIM32 antibody (ABIN2780865)

Streich, Ronchi, Connick, Haas: "Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8209-21, (2013) (PubMed).

Kudryashova, Wu, Havton, Spencer: "Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component." in: Human molecular genetics, Vol. 18, Issue 7, pp. 1353-67, (2009) (PubMed).

Target Details for TRIM32

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Alternative Name: TRIM32

Background:

Background Information: TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Gene Name: Tripartite motif containing 32

Alternative Symbols: HT2A, BBS11, TATIP, LGMD2H, LGMDR8

Protein Name: E3 ubiquitin-protein ligase TRIM32

Molecular Weight: 72kDa

Gene ID: 22954

NCBI Accession: NP_036342

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling