UBE3B antibody (Middle Region)

Catalog No. ABIN2781245

This Rabbit Polyclonal antibody specifically detects UBE3B in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for UBE3B antibody (Middle Region) (ABIN2781245)

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE3B Antibody

Binding Specificity: Middle Region

Sequence: VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1068 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE3B antibody (ABIN2781245)

Szafranski, Schindler, Taudien, Hiller, Huse, Jahn, Schreiber, Backofen, Platzer: "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns." in: Genome biology, Vol. 8, Issue 8, pp. R154, (2008) (PubMed).

Target Details for UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: UBE3B

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE3B is a member of the E3 ubiquitin-conjugating enzyme family. UBE3B may interact with other proteins and play a role in stress response.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family. The encoded protein may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
Alias Symbols: DKFZp586K2123, DKFZp686A1051, FLJ45294, MGC131858, MGC78388
Protein Interaction Partner: SHFM1, UBC, IQCB1,
Protein Size: 1068

Molecular Weight: 123 kDa

Gene ID: 89910

NCBI Accession: NM_130466, NP_569733

UniProt: Q7Z3V4