UBE3B antibody (AA 550-630)

Catalog No. ABIN7225756

The Rabbit Polyclonal anti-UBE3B antibody (ABIN7225756) specifically detects UBE3B in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for UBE3B antibody (AA 550-630) (ABIN7225756)

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-UBE3B Antibody

Binding Specificity: AA 550-630

Purpose: Rabbit Anti-UBE3B Polyclonal Antibody

Specificity: UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human UBE3B at AA range: 550-630

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: UBE3B

Background: UBE3B, Ubiquitin-protein ligase E3BThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: e1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. UBE3B (ubiquitin protein ligase E3B) encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for UBE3B.

Molecular Weight: 123kD

Gene ID: 89910

UniProt: Q7Z3V4