RNF185 antibody (Middle Region)

Catalog No. ABIN2781255

There is 1 publication for this product available. The Rabbit Polyclonal anti-RNF185 antibody is suitable to detect RNF185 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio) and Rabbit. It has been validated for WB.

Quick Overview for RNF185 antibody (Middle Region) (ABIN2781255)

Target: RNF185 (Ring Finger Protein 185 (RNF185))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF185 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF185 Antibody

Binding Specificity: Middle Region

Purpose: RNF185 Antibody - middle region

Sequence: QVCPVCKAGI SRDKVIPLYG RGSTGQQDPR EKTPPRPQGQ RPEPENRGGF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF185. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF185

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43414-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF185 antibody (ABIN2781255)

Collins, Wright, Edwards, Davis, Grinham, Cole, Goward, Aguado, Mallya, Mokrab, Huckle, Beare, Dunham: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, (2004) (PubMed).

Target Details for RNF185

Target: RNF185 (Ring Finger Protein 185 (RNF185))

Alternative Name: RNF185

Background:

Background Information: RNF185 is a multi-pass membrane protein. It contains 1 RING-type zinc finger.The exact function of RNF185 remains unknown.

Gene Name: Ring finger protein 185

Alternative Symbols: FLJ38628

Protein Name: E3 ubiquitin-protein ligase RNF185

Molecular Weight: 20kDa

Gene ID: 91445

NCBI Accession: NP_689480

UniProt: Q96GF1