RNF144B antibody (Middle Region)

Catalog No. ABIN2781285

This Rabbit Polyclonal antibody specifically detects RNF144B in WB. It exhibits reactivity toward Human, Mouse, Cow, Rat, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for RNF144B antibody (Middle Region) (ABIN2781285)

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Reactivity: Human, Mouse, Cow, Rat, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF144B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNF144B Antibody

Binding Specificity: Middle Region

Purpose: RNF144B Antibody - middle region

Sequence: KHTFCWYCLQ NLDNDIFLRH YDKGPCRNKL GHSRASVMWN RTQVVGILVG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF144B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF144B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43479-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF144B

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Alternative Name: RNF144B

Background:

Background Information: RNF144B is an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their de

Gene Name: Ring finger protein 144B

Alternative Symbols: PIR2, IBRDC2, p53RFP, bA528A10.3

Protein Name: E3 ubiquitin-protein ligase RNF144B

Molecular Weight: 34kDa

Gene ID: 255488

NCBI Accession: NP_877434

UniProt: Q7Z419