PAM antibody (N-Term)

Catalog No. ABIN2781297

The Rabbit Polyclonal anti-PAM antibody is suitable to detect PAM in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for PAM antibody (N-Term) (ABIN2781297)

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAM Antibody

Binding Specificity: N-Term

Sequence: PKGVGFRVGG ETGSKYFVLQ VHYGDISAFR DNNKDCSGVS LHLTRLPQPL

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PAM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PAM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 974 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Alternative Name: PAM

Background: PAM is a multifunctional protein. It has two enzymatically active domains with catalytic activities-peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products.This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known.
Alias Symbols: PAL, PHM
Protein Interaction Partner: UBC, CALM1, UHMK1, RASSF9, KALRN, TSC2, PRKCA, TSC1,
Protein Size: 974

Molecular Weight: 108 kDa

Gene ID: 5066

NCBI Accession: NM_000919, NP_000910

UniProt: A6NMR0