PEMT antibody (C-Term)

Catalog No. ABIN2781316

The Rabbit Polyclonal anti-PEMT antibody (ABIN2781316) specifically detects PEMT in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for PEMT antibody (C-Term) (ABIN2781316)

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PEMT Antibody

Binding Specificity: C-Term

Purpose: PEMT Antibody - C-terminal region

Sequence: GWAIMHASPT GLLLTVLVAL TYIVALLYEE PFTAEIYRQK ASGSHKRS

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 83%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 86%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against PEMT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PEMT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43558-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PEMT

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Alternative Name: PEMT

Background:

Background Information: PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene encodes PEMT protein is within the Smith-Magenis syndrome region on chromosome 17. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.

Gene Name: Phosphatidylethanolamine N-methyltransferase

Alternative Symbols: PLMT, PNMT, PEAMT, PEMPT, PEMT2

Protein Name: Phosphatidylethanolamine N-methyltransferase

Molecular Weight: 22 kDa

Gene ID: 10400

NCBI Accession: NP_680477

UniProt: Q9BW86