GLS2 antibody (Middle Region)

Catalog No. ABIN2781319

Cited in 2+ publications. The Rabbit Polyclonal anti-GLS2 antibody (ABIN2781319) specifically detects GLS2 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Pig, Zebrafish (Danio rerio), Cow, Guinea Pig and Rabbit samples.

Quick Overview for GLS2 antibody (Middle Region) (ABIN2781319)

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLS2 Antibody

Binding Specificity: Middle Region

Purpose: GLS2 Antibody - middle region

Sequence: FVGKEPSGLR YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against GLS2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GLS2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101632

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GLS2 antibody (ABIN2781319)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Toriumi, Mouri, Narusawa, Aoyama, Ikawa, Lu, Nagai, Mamiya, Kim, Nabeshima: "Prenatal NMDA receptor antagonism impaired proliferation of neuronal progenitor, leading to fewer glutamatergic neurons in the prefrontal cortex." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 37, Issue 6, pp. 1387-96, (2012) (PubMed).

Target Details for GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Alternative Name: GLS2

Background:

Background Information: GLS2 is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well.

Gene Name: Glutaminase 2 (liver, mitochondrial)

Alternative Symbols: GA, GLS, LGA, hLGA

Protein Name: Glutaminase liver isoform, mitochondrial

Molecular Weight: 36kDa

Gene ID: 27165

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, Warburg Effect