PI3 antibody (Middle Region)

Catalog No. ABIN2782187

The Rabbit Polyclonal anti-PI3 antibody (ABIN2782187) specifically detects PI3 in WB. The antibody is reactive with Human, Cow, Pig and Sheep samples.

Quick Overview for PI3 antibody (Middle Region) (ABIN2782187)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivity: Human, Cow, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PI3 Antibody

Binding Specificity: Middle Region

Purpose: PI3 Antibody - middle region

Sequence: KGRVPFNGQD PVKGQVSVKG QDKVKAQEPV KGPVSTKPGS CPIILIRCAM

Predicted Reactivity: Cow: 85%, Human: 100%, Pig: 77%, Sheep: 77%

Characteristics: This is a rabbit polyclonal antibody against PI3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PI3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45341-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: PI3

Background:

Background Information: PI3 is an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. PI3 contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. PI3 belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.This gene encodes an elastase-specific inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Peptidase inhibitor 3, skin-derived

Alternative Symbols: ESI, WAP3, SKALP, WFDC14, cementoin

Protein Name: Elafin

Molecular Weight: 6kDa

Gene ID: 5266

NCBI Accession: NP_002629

UniProt: P19957