HAL antibody (C-Term)

Catalog No. ABIN2782321

There are 2+ publications for this product available. The Rabbit Polyclonal anti-HAL antibody is suitable to detect HAL in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig and Horse. It has been validated for WB and IHC.

Quick Overview for HAL antibody (C-Term) (ABIN2782321)

Target: HAL (Histidine Ammonia-Lyase (HAL))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HAL Antibody

Binding Specificity: C-Term

Purpose: HAL Antibody - C-terminal region

Sequence: EAAHRLLLEQ KVWEVAAPYI EKYRMEHIPE SRPLSPTAFS LQFLHKKSTK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against HAL. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HAL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102553

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HAL antibody (ABIN2782321)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Alemán, Ortíz, Langley, Tovar, Torres: "Regulation by glucagon of the rat histidase gene promoter in cultured rat hepatocytes and human hepatoblastoma cells." in: American journal of physiology. Endocrinology and metabolism, Vol. 289, Issue 1, pp. E172-9, (2005) (PubMed).

Target Details for HAL

Target: HAL (Histidine Ammonia-Lyase (HAL))

Alternative Name: HAL

Background:

Background Information: HAL is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. HAL defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluidsHistidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids.

Gene Name: Histidine ammonia-lyase

Alternative Symbols: HIS, HSTD

Protein Name: Histidine ammonia-lyase

Molecular Weight: 72kDa

Gene ID: 3034

NCBI Accession: NP_002099

UniProt: P42357