GLUD1 antibody (N-Term)

Catalog No. ABIN2782330

This Rabbit Polyclonal antibody specifically detects GLUD1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Horse, Pig, Dog, Sheep, Rabbit and Guinea Pig. It has been mentioned in 2+ publications

Quick Overview for GLUD1 antibody (N-Term) (ABIN2782330)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Horse, Pig, Dog, Sheep, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLUD1 Antibody

Binding Specificity: N-Term

Purpose: GLUD1 Antibody - N-terminal region

Sequence: AKAGVKINPK NYTDNELEKI TRRFTMELAK KGFIGPGIDV PAPDMSTGER

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against GLUD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLUD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45709-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GLUD1 antibody (ABIN2782330)

Spanaki, Kotzamani, Plaitakis: "Widening Spectrum of Cellular and Subcellular Expression of Human GLUD1 and GLUD2 Glutamate Dehydrogenases Suggests Novel Functions." in: Neurochemical research, Vol. 42, Issue 1, pp. 92-107, (2016) (PubMed).

Spanaki, Kotzamani, Petraki, Drakos, Plaitakis: "Expression of human GLUD1 and GLUD2 glutamate dehydrogenases in steroid producing tissues." in: Molecular and cellular endocrinology, Vol. 415, pp. 1-11, (2015) (PubMed).

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1

Background:

Background Information: L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification.L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-51 M20867.1 1-51 52-3120 BC112946.1 16-3084

Gene Name: Glutamate dehydrogenase 1

Alternative Symbols: GDH, GDH1, GLUD

Protein Name: Glutamate dehydrogenase 1

Molecular Weight: 61 kDa

Gene ID: 2746

NCBI Accession: NP_005262

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect