CSTB antibody (N-Term)

Catalog No. ABIN2782350

The Rabbit Polyclonal anti-CSTB antibody (ABIN2782350) specifically detects CSTB in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Pig, Sheep, Rabbit and Guinea Pig samples.

Quick Overview for CSTB antibody (N-Term) (ABIN2782350)

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human, Rat, Mouse, Cow, Horse, Pig, Sheep, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CSTB Antibody

Binding Specificity: N-Term

Purpose: CSTB Antibody - N-terminal region

Sequence: MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG

Predicted Reactivity: Cow: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 79%, Rat: 86%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CSTB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CSTB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098231

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB

Background:

Background Information: CSTB is a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in CSTB gene are responsible for the primary defects in patients with progressive myoclonic epilepsy a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy.The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Gene Name: Cystatin B (stefin B)

Alternative Symbols: PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A

Protein Name: Cystatin-B

Molecular Weight: 11kDa

Gene ID: 1476

NCBI Accession: NP_000091

UniProt: P04080

Pathways: Response to Water Deprivation