PRIM1 antibody (N-Term)

Catalog No. ABIN2782431

The Rabbit Polyclonal anti-PRIM1 antibody has been validated for WB. It is suitable to detect PRIM1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio) and Saccharomyces cerevisiae.

Quick Overview for PRIM1 antibody (N-Term) (ABIN2782431)

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRIM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRIM1 Antibody

Binding Specificity: N-Term

Sequence: SQYYRWLNYG GVIKNYFQHR EFSFTLKDDI YIRYQSFNNQ SDLEKEMQKM

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 80%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PRIM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRIM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 420 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRIM1

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Alternative Name: PRIM1

Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
Alias Symbols: MGC12308, p49
Protein Interaction Partner: FAM96B, CIAO1, UBC, MMS19, MDC1, POLA2, PRIM2, POLE, POLA1, COPS6, RPA3, RPA2, RPA1, RAE1, MRPL38, TP53, XRCC5,
Protein Size: 420

Molecular Weight: 50 kDa

Gene ID: 5557

NCBI Accession: NM_000946, NP_000937

UniProt: P49642

Pathways: Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome