PRIM1 antibody (AA 340-420)

Catalog No. ABIN7216656

This Rabbit Polyclonal antibody specifically detects PRIM1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRIM1 antibody (AA 340-420) (ABIN7216656)

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PRIM1 Antibody

Binding Specificity: AA 340-420

Purpose: Rabbit Anti-PRIM1 Polyclonal Antibody

Specificity: PRIM1 Polyclonal Antibody detects endogenous levels of PRIM1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human PRIM1 at AA range: 340-420

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PRIM1

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Alternative Name: PRIM1

Background: PRIM1, DNA primase small subunit, DNA primase 49 kDa subunit, p49The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. Pimase (DNA) subunit 1 ncoded by PRIM1 is the small, 49 kDa primase subunit.

Molecular Weight: 50kD

Gene ID: 5557

UniProt: P49642

Pathways: Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome