RRM2B antibody (Middle Region)

Catalog No. ABIN2782441

The Rabbit Polyclonal anti-RRM2B antibody is suitable to detect RRM2B in samples from Human, Mouse, Rat, Cow, Rabbit, Horse, Pig, Dog, Guinea Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for RRM2B antibody (Middle Region) (ABIN2782441)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Pig, Dog, Guinea Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RRM2B Antibody

Binding Specificity: Middle Region

Purpose: RRM2B Antibody - middle region

Sequence: VHSEMYSLLI DTYIRDPKKR EFLFNAIETM PYVKKKADWA LRWIADRKST

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 100%, Rat: 93%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RRM2B. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human RRM2B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46032-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Alternative Name: RRM2B

Background:

Background Information: This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.

Gene Name: ribonucleotide reductase M2

Alternative Symbols: P53R2, MTDPS8A, MTDPS8B

Protein Name: Ribonucleoside-diphosphate reductase subunit M2 B

Molecular Weight: 32kDa

Gene ID: 50484

UniProt: Q7LG56

Pathways: p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling