SKAP1 antibody (N-Term)

Catalog No. ABIN2782452

The Rabbit Polyclonal anti-SKAP1 antibody (ABIN2782452) specifically detects SKAP1 in WB. The antibody is reactive with Human, Rat, Mouse and Rabbit samples.

Quick Overview for SKAP1 antibody (N-Term) (ABIN2782452)

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SKAP1 Antibody

Binding Specificity: N-Term

Purpose: SKAP1 Antibody - N-terminal region

Sequence: RDHILRGFQQ IKARYYWDFQ PQGGDIGQDS SDDNHSGTLG LSLTSDAPFL

Predicted Reactivity: Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SKAP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SKAP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46047-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SKAP1

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Alternative Name: SKAP1

Background:

Background Information: SKAP1 Is a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Gene Name: Src kinase associated phosphoprotein 1

Alternative Symbols: SCAP1, SKAP55, HEL-S-81p

Protein Name: Src kinase-associated phosphoprotein 1

Molecular Weight: 41kDa

Gene ID: 8631

NCBI Accession: NP_003717

UniProt: Q86WV1