RFC3 antibody (C-Term)

Catalog No. ABIN2782474

Cited in 1 publication. The Rabbit Polyclonal anti-RFC3 antibody (ABIN2782474) specifically detects RFC3 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Pig samples.

Quick Overview for RFC3 antibody (C-Term) (ABIN2782474)

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFC3 Antibody

Binding Specificity: C-Term

Sequence: IIMKGLLSEL LHNCDGQLKG EVAQMAAYYE HRLQLGSKAI YHLEAFVAKF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RFC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RFC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 356 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RFC3 antibody (ABIN2782474)

You, Li, Kim, Kang, Koh, Chwae, Kim, Kim, Park, Jang, Kim: "Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors." in: PLoS biology, Vol. 5, Issue 5, pp. e109, (2007) (PubMed).

Target Details for RFC3

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Alternative Name: RFC3

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: MGC5276, RFC38
Protein Interaction Partner: PCNA, TP53, UBC, RPA3, RPA2, RPA1, RPP40, FBXO6, ARFGAP1, VCP, RFC4, RFC5, RFC2, RFC1, PURB, PURA, MCM3, Cebpb, RAD21, HDGF, SMARCAD1, EBNA-LP, MESDC2, MYC, FAN1, MEPCE, USP20, CHTF8, RAD17, BRD4, CHTF18,
Protein Size: 356

Molecular Weight: 40 kDa

Gene ID: 5983

NCBI Accession: NM_002915, NP_002906

UniProt: P40938

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA