PDK3 antibody (N-Term)

Catalog No. ABIN2782501

The Rabbit Polyclonal anti-PDK3 antibody has been validated for WB and IHC. It is suitable to detect PDK3 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Dog, Horse, Rabbit and Pig.

Quick Overview for PDK3 antibody (N-Term) (ABIN2782501)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Dog, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDK3 Antibody

Binding Specificity: N-Term

Sequence: RPSVGLVQSW YMQSFLELLE YENKSPEDPQ VLDNFLQVLI KVRNRHNDVV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against PDK3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PDK3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Alternative Name: PDK3

Background: PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Alias Symbols: -
Protein Interaction Partner: APP, UBD, UBC, ICT1, EGFR, DLAT, PDHA1,
Protein Size: 406

Molecular Weight: 47 kDa

Gene ID: 5165

NCBI Accession: NM_005391, NP_005382

UniProt: Q15120

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect