PFAS antibody (N-Term)

Catalog No. ABIN2782529

Product Details anti-PFAS Antibody

Target: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFAS antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ESIMSTQESS NPNNVLKFCD NSSAIQGKEV RFLRPEDPTR PSRFQQQQGL

Predicted Reactivity: Cow: 83%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 79%

Characteristics: This is a rabbit polyclonal antibody against PFAS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PFAS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1338 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PFAS antibody (ABIN2782529)

French, Zhao, An, Niessen, Deng, Cravatt, Benkovic: "Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2528-33, (2013) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for PFAS

Target: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Alternative Name: PFAS (PFAS Products)

Background: Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.
Alias Symbols: FGAMS, FGARAT, KIAA0361, PURL
Protein Interaction Partner: UBC, SUMO2, TNRC6B, GINS3, ISOC1, BZW2, DCPS, MAT2B, ABHD14A, ERP44, PDCD10, FERMT2, NPEPPS, CNBP, YWHAZ, VCL, RAP1GDS1, PAK2, MSN, LDHB, HSPA9, EIF5, EEF2, ECHS1, DFFA, CKB, CAPN1, SGTA, XIAP, UBR1, USP28, PPP6R3, ZNF593, SEPHS1, CHERP, PRDX6, USP14, UBD
Protein Size: 1338

Molecular Weight: 145 kDa

Gene ID: 5198

NCBI Accession: NM_012393, NP_036525

UniProt: O15067