MELK antibody (N-Term)

Catalog No. ABIN2782539

This Rabbit Polyclonal antibody specifically detects MELK in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Guinea Pig, Dog, Rabbit and Pig.

Quick Overview for MELK antibody (N-Term) (ABIN2782539)

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig, Dog, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MELK Antibody

Binding Specificity: N-Term

Sequence: GFAKVKLACH VLTGEMVAIK IMDKNALGSD LPRVKTEIDA LKSLRHQHIC

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against Melk. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Melk

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: Melk

Background: Melk is a Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity, phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Melk Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It Plays a key role in cell proliferation. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. It's also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus and may also play a role in primitive hematopoiesis.
Alias Symbols: AI327312, MPK38, mKIAA0175
Protein Interaction Partner: Txn1, ZNF622, UBC, TXN, TP53, MAP3K5, MDM2, SMAD7, SMAD3,
Protein Size: 643

Molecular Weight: 70 kDa

Gene ID: 17279

NCBI Accession: NM_010790, NP_034920

UniProt: Q61846