NMT1 antibody (N-Term)

Catalog No. ABIN2782574

Cited in 1 publication. The Rabbit Polyclonal anti-NMT1 antibody (ABIN2782574) specifically detects NMT1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Pig, Rabbit, Zebrafish (Danio rerio), Dog and Horse samples.

Quick Overview for NMT1 antibody (N-Term) (ABIN2782574)

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Pig, Rabbit, Zebrafish (Danio rerio), Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NMT1 Antibody

Binding Specificity: N-Term

Purpose: NMT1 Antibody - N-terminal region

Sequence: TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NMT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NMT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46299-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NMT1 antibody (ABIN2782574)

Takamune, Gota, Misumi, Tanaka, Okinaka, Shoji: "HIV-1 production is specifically associated with human NMT1 long form in human NMT isozymes." in: Microbes and infection / Institut Pasteur, Vol. 10, Issue 2, pp. 143-50, (2008) (PubMed).

Target Details for NMT1

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Alternative Name: NMT1

Background:

Background Information: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1).

Gene Name: N-myristoyltransferase 1

Alternative Symbols: NMT

Protein Name: Glycylpeptide N-tetradecanoyltransferase 1

Molecular Weight: 57kDa

Gene ID: 4836

NCBI Accession: NP_066565

UniProt: P30419

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling