SPTLC1 antibody (Middle Region)

Catalog No. ABIN2782713

The Rabbit Polyclonal anti-SPTLC1 antibody is suitable to detect SPTLC1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for SPTLC1 antibody (Middle Region) (ABIN2782713)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPTLC1 Antibody

Binding Specificity: Middle Region

Purpose: SPTLC1 Antibody - middle region

Sequence: ICPLPELVKL KYKYKARIFL EESLSFGVLG EHGRGVTEHY GINIDDIDLI

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SPTLC1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPTLC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111351

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1

Background:

Background Information: Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1.Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Gene Name: Serine palmitoyltransferase, long chain base subunit 1

Alternative Symbols: HSN1, LBC1, LCB1, SPT1, SPTI, HSAN1

Protein Name: Serine palmitoyltransferase 1

Molecular Weight: 53 kDa

Gene ID: 10558

NCBI Accession: NP_006406

UniProt: O15269