SPTLC1 antibody (C-Term)

Catalog No. ABIN389109

Cited in 2+ publications. The Rabbit Polyclonal anti-SPTLC1 antibody (Clone RB4727) (ABIN389109) specifically detects SPTLC1 in WB. The antibody is reactive with Human samples.

Quick Overview for SPTLC1 antibody (C-Term) (ABIN389109)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB4727

Product Details anti-SPTLC1 Antibody

Binding Specificity: AA 405-437, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPTLC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-437 amino acids from the C-terminal region of human SPTLC1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SPTLC1 antibody (ABIN389109)

Wei, Yerokun, Leipelt, Haynes, Radhakrishna, Momin, Kelly, Park, Wang, Carton, Uhlinger, Merrill: "Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology." in: Biochimica et biophysica acta, Vol. 1791, Issue 8, pp. 746-56, (2009) (PubMed).

Medler, Petrusca, Lee, Hubbard, Berdyshev, Skirball, Kamocki, Schuchman, Tuder, Petrache: "Apoptotic sphingolipid signaling by ceramides in lung endothelial cells." in: American journal of respiratory cell and molecular biology, Vol. 38, Issue 6, pp. 639-46, (2008) (PubMed).

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1

Background: Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.

Molecular Weight: 52744

Gene ID: 10558

NCBI Accession: NP_001268232, NP_006406, NP_847894

UniProt: O15269