EBP antibody (N-Term)

Catalog No. ABIN2782722

This Rabbit Polyclonal antibody specifically detects EBP in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Pig and Rabbit.

Quick Overview for EBP antibody (N-Term) (ABIN2782722)

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Reactivity: Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EBP Antibody

Binding Specificity: N-Term

Purpose: EBP Antibody - N-terminal region

Sequence: LVIEGWFVLY YEDLLGDQAF LSQLWKEYAK GDSRYILGDN FTVCMETITA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 85%, Pig: 93%, Rabbit: 93%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against EBP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EBP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099584

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EBP

Target: EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))

Alternative Name: EBP

Background:

Background Information: EBP is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in EBP gene cause Chondrodysplasia punctata 2 (CDPX2, also known as Conradi-Hunermann syndrome).Emopamil-binding protein (EBP) is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. EBP shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of EBP is its high content of aromatic amino acid residues (>23 % ) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2, also known as Conradi-Hunermann syndrome).

Gene Name: Emopamil binding protein (sterol isomerase)

Alternative Symbols: CPX, CHO2, CPXD, MEND, CDPX2

Protein Name: 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase

Molecular Weight: 26kDa

Gene ID: 10682

NCBI Accession: NP_006570

UniProt: Q15125