CHST15 antibody (Middle Region)

Catalog No. ABIN405878

The Rabbit Polyclonal anti-CHST15 antibody is suitable to detect CHST15 in samples from Human, Rat, Mouse, Dog, Guinea Pig, Horse, Cow and Rabbit. It has been validated for WB.

Quick Overview for CHST15 antibody (Middle Region) (ABIN405878)

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHST15 Antibody

Binding Specificity: Middle Region

Sequence: YDNSTDGEPP FLTQDFIHAF QPNARLIVML RDPVERLYSD YLYFASSNKS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GALNAC4S-6ST. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GALNAC4S-6ST

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 561 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHST15

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Alternative Name: GALNAC4S-6ST

Background: GALNAC4S-6ST is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. GALNAC4S-6ST may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression, however such results are unclear in vivo.
Alias Symbols: BRAG, DKFZp781H1369, KIAA0598, MGC34346, RP11-47G11.1, GALNAC4S-6ST
Protein Interaction Partner: UBC, RRAGD, LYN, HCK,
Protein Size: 561

Molecular Weight: 65 kDa

Gene ID: 51363

NCBI Accession: NM_015892, NP_056976

UniProt: Q7LFX5

Pathways: Glycosaminoglycan Metabolic Process