Transglutaminase 2 antibody (Middle Region)

Catalog No. ABIN2782978

The Rabbit Polyclonal anti-Transglutaminase 2 antibody is suitable to detect Transglutaminase 2 in samples from Human, Mouse, Rat, Rabbit, Guinea Pig, Dog, Horse, Cow and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for Transglutaminase 2 antibody (Middle Region) (ABIN2782978)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Dog, Horse, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Transglutaminase 2 Antibody

Binding Specificity: Middle Region

Purpose: TGM2 Antibody - middle region

Sequence: VGRDEREDIT HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG

Predicted Reactivity: Cow: 91%, Dog: 91%, Guinea Pig: 93%, Horse: 91%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against TGM2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TGM2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8114270

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2

Background:

Background Information: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, TGM2 is the autoantigen implicated in celiac disease.Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)

Alternative Symbols: TGC, tTG, G(h), hTG2, TG(C)

Protein Name: Protein-glutamine gamma-glutamyltransferase 2

Molecular Weight: 77 kDa

Gene ID: 7052

NCBI Accession: NP_945189

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling