Transglutaminase 2 antibody (N-Term)

Catalog No. ABIN2782979

Cited in 1 publication. The Rabbit Polyclonal anti-Transglutaminase 2 antibody (ABIN2782979) specifically detects Transglutaminase 2 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Guinea Pig, Dog, Horse, Cow and Pig samples.

Quick Overview for Transglutaminase 2 antibody (N-Term) (ABIN2782979)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Dog, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Transglutaminase 2 Antibody

Binding Specificity: N-Term

Purpose: TGM2 Antibody - N-terminal region

Sequence: MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW LTLHFEGRNY

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against TGM2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TGM2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8114271

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Transglutaminase 2 antibody (ABIN2782979)

Guise, Engel, Williams, Mahdi, Bidwell, Chade: "Biopolymer-delivered vascular endothelial growth factor improves renal outcomes following revascularization." in: American journal of physiology. Renal physiology, Vol. 316, Issue 5, pp. F1016-F1025, (2019) (PubMed).

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2

Background:

Background Information: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, TGM2 is the autoantigen implicated in celiac disease.Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)

Alternative Symbols: TGC, tTG, G(h), hTG2, TG(C)

Protein Name: Protein-glutamine gamma-glutamyltransferase 2

Molecular Weight: 77kDa

Gene ID: 7052

NCBI Accession: NP_004604

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling