FTO antibody (Middle Region)

Catalog No. ABIN2782981

The Rabbit Polyclonal anti-FTO antibody is suitable to detect FTO in samples from Human, Mouse, Rat, Dog, Rabbit, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for FTO antibody (Middle Region) (ABIN2782981)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FTO Antibody

Binding Specificity: Middle Region

Purpose: FTO Antibody - middle region

Sequence: WWCQPMAQLE ALWKKMEGVT NAVLHEVKRE GLPVEQRNEI LTAILASLTA

Predicted Reactivity: Dog: 86%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against FTO. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FTO

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47474-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO

Background:

Background Information: The exact function of this gene is not know. Studies in mice suggest that it may be involved in nucleic acid demethylation, and that its mRNA level is regulated by feeding and fasting. Genomewide association studies of type 2 diabetes indicate this gene as a diabetes susceptibility locus. Mutation in this gene has been associated with growth retardation, developmental delay, coarse facies, and early death.

Gene Name: Fat mass and obesity associated

Alternative Symbols: GDFD, ALKBH9, BMIQ14

Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO

Molecular Weight: 58kDa

Gene ID: 79068

NCBI Accession: NP_001073901

UniProt: Q9C0B1