NCKAP1L antibody (N-Term)

Catalog No. ABIN2783034

This Rabbit Polyclonal antibody specifically detects NCKAP1L in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Rat, Horse, Rabbit, Guinea Pig and Pig.It has been mentioned in 1 publication

Quick Overview for NCKAP1L antibody (N-Term) (ABIN2783034)

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Reactivity: Human, Mouse, Cow, Dog, Rat, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1L antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCKAP1L Antibody

Binding Specificity: N-Term

Purpose: NCKAP1L Antibody - N-terminal region

Sequence: CSDPKSKPPF LLEKSMEPSL KYINKKFPNI DVRNSTQHLG PVHREKAEII

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NCKAP1L. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NCKAP1L

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47584-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NCKAP1L antibody (ABIN2783034)

Weiner, Marganski, Wu, Altschuler, Kirschner: "An actin-based wave generator organizes cell motility." in: PLoS biology, Vol. 5, Issue 9, pp. e221, (2007) (PubMed).

Target Details for NCKAP1L

Target: NCKAP1L (NCK-Associated Protein 1-Like (NCKAP1L))

Alternative Name: NCKAP1L

Background:

Background Information: NCKAP1L is a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells, while hematopoietic protein 2 is preferentially expressed in brain, heart, liver and testis. The function of the HEM1 product has not been established but it is thought to play an essential role in oogenesis.The protein encoded by this gene is a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells, while hematopoietic protein 2 is preferentially expressed in brain, heart, liver and testis. The function of the HEM1 product has not been established but it is thought to play an essential role in oogenesis.

Gene Name: NCK-associated protein 1-like

Alternative Symbols: HEM1, IMD72

Protein Name: Nck-associated protein 1-like

Molecular Weight: 128kDa

Gene ID: 3071

NCBI Accession: NP_005328

UniProt: P55160

Pathways: Regulation of Actin Filament Polymerization