NDE1 antibody (Middle Region)

Catalog No. ABIN2783161

There is 1 publication for this product available. The Rabbit Polyclonal anti-NDE1 antibody is suitable to detect NDE1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for NDE1 antibody (Middle Region) (ABIN2783161)

Target: NDE1

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDE1 Antibody

Binding Specificity: Middle Region

Purpose: NDE1 Antibody - middle region

Sequence: AHRGPSSSLN TPGSFRRGLD DSTGGTPLTP AARISALNIV GDLLRKVGAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against NDE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NDE1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47932-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NDE1 antibody (ABIN2783161)

Burdick, Kamiya, Hodgkinson, Lencz, DeRosse, Ishizuka, Elashvili, Arai, Goldman, Sawa, Malhotra: "Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding." in: Human molecular genetics, Vol. 17, Issue 16, pp. 2462-73, (2008) (PubMed).

Target Details for NDE1

Target: NDE1

Alternative Name: NDE1

Background:

Background Information: NDE1 is required for centrosome duplication and formation and function of the mitotic spindle. It is essential for the development of the cerebral cortex. NDE1 may regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex

Gene Name: NudE nuclear distribution E homolog 1 (A. nidulans)

Alternative Symbols: NDE, LIS4, MHAC, NUDE, NUDE1, HOM-TES-87

Protein Name: Nuclear distribution protein nudE homolog 1

Molecular Weight: 38kDa

Gene ID: 54820

NCBI Accession: NP_060138

UniProt: Q9NXR1