ARF1 antibody (Middle Region)

Catalog No. ABIN2783267

This Rabbit Polyclonal antibody specifically detects ARF1 in WB, IHC and IP. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Sheep, Rabbit, Guinea Pig, Horse and Saccharomyces cerevisiae.

Quick Overview for ARF1 antibody (Middle Region) (ABIN2783267)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Sheep, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ARF1 Antibody

Binding Specificity: Middle Region

Sequence: MRMLAEDELR DAVLLVFANK QDLPNAMNAA EITDKLGLHS LRHRNWYIQA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast 100%

Characteristics: This is a rabbit polyclonal antibody against ARF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 181 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF family. The family is composed of small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. These protein, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Alias Symbols: -
Protein Interaction Partner: GGA1, GGA3, GGA2, HUWE1, UBC, FBXO6, MMS19, TMEM106A, VCAM1, ITGA4, ATF2, ARF3, UBD, GRK5, CDK2, HERC1, Proser1, AI837181, Kif1c, Bach1, nef, COPB1, GEA1, WBP11, TMED2, PICK1, NOA1, TMED10, EEF1G, PLEKHA8, COPG1, ARFIP1, ARFIP2, ARHGAP21, ARFGAP1, AP3D1,
Protein Size: 181

Molecular Weight: 21 kDa

Gene ID: 375

NCBI Accession: NM_001658, NP_001649

UniProt: P61204

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process