Atp5f1b (N-Term) antibody

Catalog No. ABIN2783268

Cited in 3+ publications. The Rabbit Polyclonal anti-Atp5f1b antibody (ABIN2783268) specifically detects Atp5f1b in WB and IHC. The antibody is reactive with Mouse, Human, Rat, Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Rabbit, Horse, Dog and Cow samples.

Quick Overview for Atp5f1b (N-Term) antibody (ABIN2783268)

Target: Atp5f1b

Reactivity: Mouse, Human, Rat, Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Rabbit, Horse, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: N-Term

Purpose: ATP5F1B Antibody - N-terminal region

Sequence: PIKIPVGPET LGRIMNVIGE PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ATP5B. It was validated on Western Blot and immunohistochemistry. Catalog ABIN973615 can be used a blocking peptide for this product.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP5B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48185-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References

Li, Bates, An, Terry, Wang: "Up-regulation of key microRNAs, and inverse down-regulation of their predicted oxidative phosphorylation target genes, during aging in mouse brain." in: Neurobiology of aging, Vol. 32, Issue 5, pp. 944-55, (2011) (PubMed).

Zischka, Lichtmannegger, Schmitt, Jägemann, Schulz, Wartini, Jennen, Rust, Larochette, Galluzzi, Chajes, Bandow, Gilles, DiSpirito, Esposito, Goettlicher, Summer, Kroemer: "Liver mitochondrial membrane crosslinking and destruction in a rat model of Wilson disease." in: The Journal of clinical investigation, Vol. 121, Issue 4, pp. 1508-18, (2011) (PubMed).

Guo, Prokai-Tatrai, Nguyen, Rauniyar, Ughy, Prokai: "Protein targets for carbonylation by 4-hydroxy-2-nonenal in rat liver mitochondria." in: Journal of proteomics, Vol. 74, Issue 11, pp. 2370-9, (2011) (PubMed).

Target Details

Target: Atp5f1b

Background:

Targeted Subunit: beta, mitochondrial

Background Information: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Gene Name: ATP synthase F1 subunit beta

Alternative Symbols: ATP5B, ATPMB, ATPSB, HEL-S-271

Protein Name: ATP synthase subunit beta, mitochondrial

Molecular Weight: 57 kDa

Gene ID: 506

NCBI Accession: NP_001677

UniProt: P06576