Atp5f1b (C-Term) antibody

Catalog No. ABIN2783269

This Rabbit Polyclonal antibody specifically detects Atp5f1b in WB, IHC and IP. It exhibits reactivity toward Mouse, Human, Rat, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Rabbit, Horse, Guinea Pig, Dog and Cow. It has been mentioned in 3+ publications

Quick Overview for Atp5f1b (C-Term) antibody (ABIN2783269)

Target: Atp5f1b

Reactivity: Mouse, Human, Rat, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Rabbit, Horse, Guinea Pig, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details

Binding Specificity: C-Term

Purpose: ATP5F1B Antibody - C-terminal region

Sequence: MGKLVPLKET IKGFQQILAG EYDHLPEQAF YMVGPIEEAV AKADKLAEEH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ATP5B. It was validated on Western Blot using a cell lysate as a positive control. Catalog ABIN973616 can be used a blocking peptide for this product.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48186-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References

Usui, Kajita, Kajita, Mori, Hanamoto, Ikeda, Okada, Taguchi, Kitada, Morita, Sasaki, Kitamura, Sato, Kojima, Ishizuka: "Elevated mitochondrial biogenesis in skeletal muscle is associated with testosterone-induced body weight loss in male mice." in: FEBS letters, Vol. 588, Issue 10, pp. 1935-41, (2014) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." in: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." in: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Target Details

Target: Atp5f1b

Background:

Targeted Subunit: beta, mitochondrial

Background Information: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Gene Name: ATP synthase F1 subunit beta

Alternative Symbols: ATP5B, ATPMB, ATPSB, HEL-S-271

Protein Name: ATP synthase subunit beta, mitochondrial

Molecular Weight: 57 kDa

Gene ID: 506

NCBI Accession: NP_001677

UniProt: P06576