Vimentin antibody (C-Term)

Catalog No. ABIN2783289

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB and IHC. It is suitable to detect Vimentin in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Horse, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for Vimentin antibody (C-Term) (ABIN2783289)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Horse, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Vimentin Antibody

Binding Specificity: C-Term

Sequence: SSLNLRETNL DSLPLVDTHS KRTLLIKTVE TRDGQVINET SQHHDDLE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against VIM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human VIM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 466 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Background: Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ36605
Protein Interaction Partner: PKN1, PPL, PLA2G2A, PA2G4, KRT20, TXLNB, CWF19L2, PNMA5, UBC, TP53, CHFR, SPRTN, SUMO2, SUMO3, TRIM68, STAU1, ASB15, ASB9, ASB16, ASB2, RNF2, EEF1D, SERPINH1, COPS8, FOXK1, YWHAQ, PARK2, FBXO6, CASP7, CASP3, KAT7, UBD, PINK1, BAG3, GSK3B, PAN2, CAPN1, SVI
Protein Size: 466

Molecular Weight: 54 kDa

Gene ID: 7431

NCBI Accession: NM_003380, NP_003371

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis