Selenoprotein F antibody (Middle Region)

Catalog No. ABIN2783300

This Rabbit Polyclonal antibody specifically detects Selenoprotein F in WB. It exhibits reactivity toward Cow, Human, Mouse, Pig, Rat and Zebrafish (Danio rerio).

Quick Overview for Selenoprotein F antibody (Middle Region) (ABIN2783300)

Target: Selenoprotein F (SELENOF)

Reactivity: Cow, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Selenoprotein F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Selenoprotein F Antibody

Binding Specificity: Middle Region

Purpose: SELENOF Antibody - middle region

Sequence: SDKPKLFRGL QIKYVRGSDP VLKLLDDNGN IAEELSILKW NTDSVEEFLS

Predicted Reactivity: Cow: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SEP15. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SEP15

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111288

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Selenoprotein F

Target: Selenoprotein F (SELENOF)

Alternative Name: SELENOF

Background:

Background Information: The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. It has also been suggested to have a role in cancer etiology. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Gene Name: selenoprotein F

Alternative Symbols: 45184

Protein Name: selenoprotein F

Molecular Weight: 15kDa

Gene ID: 9403

NCBI Accession: NP_004252

UniProt: O60613