MDH2 antibody (C-Term)

Catalog No. ABIN2783318

The Rabbit Polyclonal anti-MDH2 antibody has been validated for WB. It is suitable to detect MDH2 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit and Zebrafish (Danio rerio). There are 3+ publications available.

Quick Overview for MDH2 antibody (C-Term) (ABIN2783318)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MDH2 Antibody

Binding Specificity: C-Term

Sequence: TYFSTPLLLG KKGIEKNLGI GKVSSFEEKM ISDAIPELKA SIKKGEDFVK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against MDH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MDH2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 338 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MDH2 antibody (ABIN2783318)

Kwon, Rahman, Ryu, Khatun, Pang: "Comparison of markers predicting litter size in different pig breeds." in: Andrology, Vol. 5, Issue 3, pp. 568-577, (2017) (PubMed).

Freund, Prenni, Curthoys: "Response of the mitochondrial proteome of rat renal proximal convoluted tubules to chronic metabolic acidosis." in: American journal of physiology. Renal physiology, Vol. 304, Issue 2, pp. F145-55, (2013) (PubMed).

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: MDH2

Background: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: M-MDH, MDH, MGC:3559, MOR1
Protein Interaction Partner: FUS, HUWE1, GRSF1, UBC, MDM2, PARK2, UBD, METTL21B, VCAM1, ITGA4, FN1, ATF2, LDHAL6A, MRPS28, IDH1, CLNS1A, SERPINH1, ATP5B, ATP5A1, CRYAB, COPS5, CDK2, PSMA3, PCNA, HDAC5, Mapk13, PSMD4, PC, WARS, NDUFS1, MDH2, GOT2, FH, CS,
Protein Size: 338

Molecular Weight: 33 kDa

Gene ID: 4191

NCBI Accession: NM_005918, NP_005909

UniProt: P40926