POLR1D antibody (Middle Region)

Catalog No. ABIN2783504

The Rabbit Polyclonal anti-POLR1D antibody (ABIN2783504) specifically detects POLR1D in WB. The antibody is reactive with Human, Cow, Dog and Rabbit samples.

Quick Overview for POLR1D antibody (Middle Region) (ABIN2783504)

Target: POLR1D (Polymerase (RNA) I Polypeptide D, 16kDa (POLR1D))

Reactivity: Human, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1D antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1D Antibody

Binding Specificity: Middle Region

Purpose: POLR1D Antibody - middle region

Sequence: TRGTLPAVEP FQRGLNELMN VCQHVLDKFE ASIKDYKDQK ASRNESTF

Predicted Reactivity: Cow: 86%, Dog: 93%, Human: 100%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against POLR1D. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR1D

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108540

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1D

Target: POLR1D (Polymerase (RNA) I Polypeptide D, 16kDa (POLR1D))

Alternative Name: POLR1D

Background:

Targeted Subunit: RPAC2

Background Information: POLR1D belongs to the archaeal rpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR1D is the common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs.

Gene Name: Polymerase (RNA) I polypeptide D, 16 kDa

Alternative Symbols: AC19, RPA9, TCS2, RPA16, RPAC2, RPC16, POLR1C, RPO1-3

Protein Name: DNA-directed RNA polymerases I and III subunit RPAC2

Molecular Weight: 15kDa

Gene ID: 51082

NCBI Accession: NP_057056

UniProt: Q9Y2S0