POLK antibody (Middle Region)

Catalog No. ABIN2783506

This Rabbit Polyclonal antibody specifically detects POLK in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig and Rabbit.

Quick Overview for POLK antibody (Middle Region) (ABIN2783506)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLK Antibody

Binding Specificity: Middle Region

Purpose: POLK Antibody - middle region

Sequence: ATECTLEKTD KDKFVKPLEM SHKKSFFDKK RSERKWSHQD TFKCEAVNKQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108508

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: POLK

Background:

Background Information: POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Gene Name: Polymerase (DNA directed) kappa

Alternative Symbols: DINP, POLQ, DINB1

Protein Name: DNA polymerase kappa

Molecular Weight: 99kDa

Gene ID: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: DNA Damage Repair