SHMT1 antibody (N-Term)

Catalog No. ABIN2783618

The Rabbit Polyclonal anti-SHMT1 antibody (ABIN2783618) specifically detects SHMT1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Sheep, Rabbit, Cow, Horse and Saccharomyces cerevisiae samples.

Quick Overview for SHMT1 antibody (N-Term) (ABIN2783618)

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Sheep, Rabbit, Cow, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHMT1 Antibody

Binding Specificity: N-Term

Purpose: SHMT1 Antibody - N-terminal region

Sequence: NIIKKESNRQ RVGLELIASE NFASRAVLEA LGSCLNNKYS EGYPGQRYYG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SHMT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SHMT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP49040-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SHMT1

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Alternative Name: SHMT1

Background:

Background Information: This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Gene Name: Serine hydroxymethyltransferase 1 (soluble)

Alternative Symbols: SHMT, CSHMT

Protein Name: Serine hydroxymethyltransferase, cytosolic

Molecular Weight: 53kDa

Gene ID: 6470

NCBI Accession: NP_004160

UniProt: Q5RFK5