NEK3 antibody (N-Term)

Catalog No. ABIN2783629

Product Details anti-NEK3 Antibody

Target: NEK3 (NIMA related kinase 3 (NEK3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FTQMCLGVNH IHKKRVLHRD IKSKNIFLTQ NGKVKLGDFG SARLLSNPMA

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Yeast: 79%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NEK3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NEK3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 506 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NEK3

Target: NEK3 (NIMA related kinase 3 (NEK3))

Alternative Name: NEK3 (NEK3 Products)

Synonyms: HSPK36 antibody, ATNEK3 antibody, NIMA-RELATED KINASE3 antibody, NIMA-related kinase 3 antibody, T8M17.60 antibody, T8M17_60 antibody, NIMA related kinase 3 antibody, NIMA (never in mitosis gene a)-related expressed kinase 3 antibody, NIMA-related kinase 3 S homeolog antibody, NIMA-related kinase 3 antibody, NEK3 antibody, Nek3 antibody, nek3.S antibody

Background: NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.
Alias Symbols: HSPK36, MGC29949
Protein Interaction Partner: HDAC6, NMNAT1, CDCA8, BPGM, VAV2, PRLR, VAV1, CSN2,
Protein Size: 506

Molecular Weight: 58 kDa

Gene ID: 4752

NCBI Accession: NM_002498, NP_002489

UniProt: P51956