MDM1 antibody (N-Term)

Catalog No. ABIN2783764

The Rabbit Polyclonal anti-MDM1 antibody is suitable to detect MDM1 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for MDM1 antibody (N-Term) (ABIN2783764)

Target: MDM1 (Mdm1 Nuclear Protein (MDM1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MDM1 Antibody

Binding Specificity: N-Term

Purpose: MDM1 Antibody - N-terminal region

Sequence: GLRSDQLGIT KEPSFISKRR VPYHDPQISK SLEWNGAISE SNVVASPEPE

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against MDM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MDM1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104974

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MDM1

Target: MDM1 (Mdm1 Nuclear Protein (MDM1))

Alternative Name: MDM1

Background:

Background Information: MDM1 is a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage.

Gene Name: Mdm1 nuclear protein homolog (mouse)

Alternative Symbols: -

Protein Name: cDNA, FLJ95264, highly similar to Homo sapiens Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse) (MDM1), transcript variant 2, mRNA EMBL BAG37069.1

Molecular Weight: 25kDa

Gene ID: 56890

NCBI Accession: NP_064513

UniProt: B2RB22