SSX4B antibody (Synovial Sarcoma, X Breakpoint 4B) (Middle Region)

Details for Product anti-SSX4B Antibody No. ABIN2784178
Target
Binding Specificity
Middle Region
1
Reactivity
Human
1
Host
Rabbit
1
Clonality
Polyclonal
Application
Western Blotting (WB)
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human SSX4B
Blocking Peptide Blocking peptide for this product available: ABIN984477
Sequence MRSKRAADFH GNDFGNDRNH RNQVERPQMT FGSLQRIFPK IMPKKPAEEE
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against SSX4B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Target
Alternative Name SSX4B (SSX4B Antibody Abstract)
Background The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X,18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4B, represents the more centromeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.
Alias Symbols: SSX4, CT5.4
Protein Interaction Partner: HIF1A,
Protein Size: 188
Molecular Weight 22 kDa
Gene ID 548313
NCBI Accession NM_001034832, NP_001030004
UniProt O60224
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 188 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Images
Image no. 1 for anti-Synovial Sarcoma, X Breakpoint 4B (SSX4B) (Middle Region) antibody (ABIN2784178) WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive...