EIF3H antibody (N-Term)

Catalog No. ABIN2784203

There is 1 publication for this product available. The Rabbit Polyclonal anti-EIF3H antibody is suitable to detect EIF3H in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig and Pig. It has been validated for WB and IHC.

Quick Overview for EIF3H antibody (N-Term) (ABIN2784203)

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF3H Antibody

Binding Specificity: N-Term

Purpose: EIF3H Antibody - N-terminal region

Sequence: MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF3H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50491-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF3H antibody (ABIN2784203)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EIF3H

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Alternative Name: EIF3H

Background:

Targeted Subunit: H

Background Information: EIF3H belongs to the peptidase M67 family. It contains 1 MPN (JAB/Mov34) domain. EIF3H binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. It associates with the p170 subunit of EIF3.

Gene Name: Eukaryotic translation initiation factor 3, subunit H

Alternative Symbols: EIF3S3, eIF3-p40, eIF3-gamma

Protein Name: Eukaryotic translation initiation factor 3 subunit H

Molecular Weight: 40kDa

Gene ID: 8667

NCBI Accession: NP_003747

UniProt: O15372

Pathways: Ribonucleoprotein Complex Subunit Organization