MED25 antibody (N-Term)

Catalog No. ABIN2784291

This Rabbit Polyclonal antibody specifically detects MED25 in WB and ChIP. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for MED25 antibody (N-Term) (ABIN2784291)

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED25 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED25 Antibody

Binding Specificity: N-Term

Purpose: MED25 Antibody - N-terminal region

Sequence: EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50698-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MED25 antibody (ABIN2784291)

Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, (2013) (PubMed).

Target Details for MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Alternative Name: MED25

Background:

Targeted Subunit: 25

Background Information: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.

Gene Name: Mediator complex subunit 25

Alternative Symbols: P78, ACID1, ARC92, BVSYS, PTOV2, CMT2B2, TCBAP0758

Protein Name: Mediator of RNA polymerase II transcription subunit 25

Molecular Weight: 78 kDa

Gene ID: 81857

NCBI Accession: NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response