Aspartate beta Hydroxylase antibody (N-Term)

Catalog No. ABIN2784533

The Rabbit Polyclonal anti-Aspartate beta Hydroxylase antibody is suitable to detect Aspartate beta Hydroxylase in samples from Human, Mouse, Rat, Dog, Horse, Pig and Rabbit. It has been validated for WB and IHC.

Quick Overview for Aspartate beta Hydroxylase antibody (N-Term) (ABIN2784533)

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aspartate beta Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Aspartate beta Hydroxylase Antibody

Binding Specificity: N-Term

Purpose: ASPH Antibody - N-terminal region

Sequence: SEVLQGKLGI YDADGDGDFD VDDAKVLLEG PSGVAKRKTK AKVKELTKEE

Predicted Reactivity: Dog: 93%, Horse: 83%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ASPH. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASPH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8095077

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aspartate beta Hydroxylase

Target: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Alternative Name: ASPH

Background:

Background Information: ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.This gene is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.

Gene Name: Aspartate beta-hydroxylase

Alternative Symbols: AAH, BAH, HAAH, JCTN, FDLAB, junctin, CASQ2BP1

Protein Name: Aspartyl/asparaginyl beta-hydroxylase

Molecular Weight: 25kDa

Gene ID: 444

NCBI Accession: NP_064549

UniProt: Q9NRI0

Pathways: Positive Regulation of Endopeptidase Activity