HNF1A antibody (N-Term)

Catalog No. ABIN2784536

This Rabbit Polyclonal antibody specifically detects HNF1A in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse and Cowand has been mentioned in 1 publication.

Quick Overview for HNF1A antibody (N-Term) (ABIN2784536)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HNF1A Antibody

Binding Specificity: N-Term

Sequence: HAGQGGLIEE PTGDELPTKK GRRNRFKWGP ASQQILFQAY ERQKNPSKEE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HNF1A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HNF1A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 631 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HNF1A antibody (ABIN2784536)

Scheving, Zhang, Garcia, Wang, Stevenson, Threadgill, Russell: "Epidermal growth factor receptor plays a role in the regulation of liver and plasma lipid levels in adult male mice." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 306, Issue 5, pp. G370-81, (2014) (PubMed).

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A

Background: HNF1A is required for the expression of several liver specific genes. HNF1A binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Alias Symbols: HNF1, LFB1, MODY3, TCF1, TCF-1, HNF-1A, IDDM20
Protein Interaction Partner: RNF14, RYR3, RAC3, PCBD1, HIST2H2AB, RPSAP15, SNX14, CBX3, YWHAZ, YWHAG, YWHAE, YWHAB, VIM, VCP, HNF1A, SRSF7, RANBP1, PEBP1, LDHA, IGFBP7, HMGB1, ANXA2, ACTB, SIRT1, BCL6, EP300, CREBBP, GATA5, HNF1B, KAT2B, CDX2, HNF4A, TLE1, SRC, FOS, POU2F1, BCR, CEBP
Protein Size: 631

Molecular Weight: 67 kDa

Gene ID: 6927

NCBI Accession: NM_000545, NP_000536

UniProt: P20823

Pathways: Hormone Transport, Carbohydrate Homeostasis