HNF1A antibody (AA 214-339)

Catalog No. ABIN6940684

The Mouse Monoclonal anti-HNF1A antibody (Clone HNF1A-2087) (ABIN6940684) specifically detects HNF1A in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for HNF1A antibody (AA 214-339) (ABIN6940684)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: HNF1A-2087

Product Details anti-HNF1A Antibody

Binding Specificity: AA 214-339

Purpose: HNF1A (Pancreatic Tumor Suppressor) Antibody

Specificity: HNF1A belongs to the homeobox protein family and is an essential transcription factor for many hepatic genes involved in detoxification, homeostasis and metabolisms of glucose, lipid, steroid and amino acid. In addition, HNF1A is an important component of the transcriptional networks governing embryonic pancreas development and differentiation. as well as maintaining the growth and function of islet cells in adult. HNF1A (Hepatocyte nuclear factor 1 alpha) is a transcription factor that is known to regulate pancreatic differentiation and maintain homeostasis of endocrine pancreas. Recently, genome-wide association studies have implicatedHNF1Aas a susceptibility gene for pancreatic cancer.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 214-339) of human HNF1A protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Jurkat cells. Small Intestine or Pancreas.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A

Background:

Albumin proximal factor, Hepatic nuclear factor 1 alpha, Hepatic transcription factor 1 alpha, HNF-1A, Interferon production regulator factor, LFB1, LFB1 hepatic nuclear factor, Liver specific transcription factor LFB1, Maturity onset diabetes of the young 3 (MODY3), TCF1, Transcription factor 1 hepatic

Jurkat cells. Small Intestine or Pancreas.

Molecular Weight: 79kDa

Gene ID: 6927, 654455

UniProt: P20823

Pathways: Hormone Transport, Carbohydrate Homeostasis