ALKBH2 antibody (Middle Region)

Catalog No. ABIN2784623

This Rabbit Polyclonal antibody specifically detects ALKBH2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse and Zebrafish (Danio rerio).

Quick Overview for ALKBH2 antibody (Middle Region) (ABIN2784623)

Target: ALKBH2 (AlkB, Alkylation Repair Homolog 2 (ALKBH2))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALKBH2 Antibody

Binding Specificity: Middle Region

Purpose: ALKBH2 Antibody - middle region

Sequence: VPRKQATYGD AGLTYTFSGL TLSPKPWIPV LERIRDHVSG VTGQTFNFVL

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ALKBH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ALKBH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP51778-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ALKBH2

Target: ALKBH2 (AlkB, Alkylation Repair Homolog 2 (ALKBH2))

Alternative Name: ALKBH2

Background:

Background Information: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM].

Gene Name: AlkB, alkylation repair homolog 2 (E. coli)

Alternative Symbols: ABH2

Protein Name: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2

Molecular Weight: 29kDa

Gene ID: 121642

NCBI Accession: NP_001001655

UniProt: Q6NS38

Pathways: DNA Damage Repair