CELA1 antibody (N-Term)

Catalog No. ABIN2784732

This Rabbit Polyclonal antibody specifically detects CELA1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Horse and Guinea Pig.

Quick Overview for CELA1 antibody (N-Term) (ABIN2784732)

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CELA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CELA1 Antibody

Binding Specificity: N-Term

Purpose: ELA1 Antibody - N-terminal region

Sequence: LVLYGHSTQD LPETNARVVG GTEAGRNSWP SQISLQYRSG GSRYHTCGGT

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 77%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ELA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ELA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52007-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CELA1

Target: CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Alternative Name: ELA1

Background:

Background Information: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 (ELA1) expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-867 BC069454.1 1-867 868-952 AF120493.1 868-952

Gene Name: Chymotrypsin-like elastase family, member 1

Alternative Symbols: ELA1

Protein Name: Chymotrypsin-like elastase family member 1

Molecular Weight: 28kDa

Gene ID: 1990

NCBI Accession: NP_001962

UniProt: Q9UNI1