BCKDK antibody (N-Term)

Catalog No. ABIN2784788

This Rabbit Polyclonal antibody specifically detects BCKDK in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Rabbit and Horse.It has been mentioned in 1 publication

Quick Overview for BCKDK antibody (N-Term) (ABIN2784788)

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BCKDK Antibody

Binding Specificity: N-Term

Purpose: BCKDK Antibody - N-terminal region

Sequence: CLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ YCQLVRQLLD

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BCKDK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BCKDK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8095650

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BCKDK antibody (ABIN2784788)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for BCKDK

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK

Background:

Background Information: BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.

Gene Name: Branched chain ketoacid dehydrogenase kinase

Alternative Symbols: BDK, BCKDKD

Protein Name: [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Molecular Weight: 43kDa

Gene ID: 10295

NCBI Accession: NP_005872

UniProt: O14874

Pathways: SARS-CoV-2 Protein Interactome